Project

Part:BBa_K1728017:Design

Designed by: Yung-Li Chen, Katy Chang, Timothy Chan En Haw   Group: iGEM15_CGU_Taiwan   (2015-09-17)


IL1β toehold switch RNA sensor with T7 promoter & luciferase reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 922


Design Notes

No.


Source

IL1β toehold switch RNA sensor with T7 promoter (order from synthesis company) assembled with luciferase reporter (BBa_I712019) at the upstream.

References

Alexander A. Green, Pamela A. Silver, James J. Collins, Peng Yin Toehold Switches: De-Novo-Designed Regulators of Gene Expression Cell.2014;159:925-939